研究業績 / List of publicatiuons

2023

• 佐藤有里、荒井堅太*. （総説）インスリンスーパーファミリーに属するヒトリラキシン-２の生理機能と応用。東海大学先進生命科学研究所2022年度紀要　受理済み。

• Kenta Arai*, Haruka Toba, Nozomi Yamamoto, Mao Ito, Rumi Mikami, Modeling Type-1 Iodothyronine Deiodinase with Peptide-Based Aliphatic Diselenides: Potential Role of Highly Conserved His and Cys Residues as a General Acid Catalyst. Chem. Eur. J, 2023, 29, e202202387 (DOI: 10.1002/chem.202202387) (Selected as Hot Paper)

• Small Organoselenium Catalysts as a Potential Manipulator for Redox Homeostasis and Proteostasis. Kenta Arai, Chapter 25 in Chalcogen Chemistry: Fundamentals and Applications, edited by V. Lippolis, C. Santi, Eder J. L. and A. L. Braga. The Royal Society of Chemistry, pp. 648- 65 (DOI: 10.1039/BK9781839167386-00648).

 

2022

• Kenta Arai*, Rumi Mikami, Redox Chemistry of Selenols and Diselenides as Potential Manipulators for Structural Maturation of Peptides and Proteins, Metallomics Res., 2022, 2, rev1-17. (Open Access)

• Rumi Mikami, Shunsuke Tsukagoshi, Yoshiki Oda, Kenta Arai*,  S-Denitrosylase-like Activity of Cyclic Diselenides Conjugated with Xaa-His Dipeptide: Role of Proline Spacer as a Key Activity Booster. ChemBioChem, 2022, e202100394. (DOI: 10.1002/cbic.202100394) (Selected as front cover picture)

 

2021

• Rumi Mikami, Shunsuke Tsukagoshi, Kenta Arai*, Abnormal Enhancement of Protein Disulfide Isomerase-like Activity of a Cyclic Diselenide Conjugated with a Basic Amino Acid by Inserting a Glycine Spacer. Biology, 2021, 10, 1090. (DOI: 10.3390/biology10111090)

• Kenta Arai*, Yuumi Sato, Ikumi Nakajima, Manami Saito, Moeka Sasaki, Akiko Kanamori, Michio Iwaoka, Glutathione peroxidase-like functions of 1,2-diselenane-4,5-diol and its amphiphilic derivatives: Switchable catalytic cycles depending on peroxide substrates, Bioorg. Med. Chem., 2021, 29, 115866.

• Kenta Arai, Michio Iwaoka* Flexible Folding: Disulfide-Containing Peptides and Proteins Choose the Pathway Depending on the Environments, Molecules, 2021, 26(1), 195.

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2020

• Osamu Yoshino*, Yosuke Ono, Masako Honda, Kyoko Hattori, Erina Sato, Takehiro Hiraoka, Masami Ito, Mutsumi Kobayashi, Kenta Arai, Hidekazu Katayama, Hiroyoshi Tsuchida, Kaori Yamada-Nomoto, Shunsuke Iwahata, Yoshiyuki Fukushi, Shinichiro Wada, Haruko Iwase, Kaori Koga, Yutaka Osuga, Michio Iwaoka and Nobuya Unno, Relaxin-2 May Suppress Endometriosis by Reducing Fibrosis, Scar Formation, and Inflammation, Biomedicines 2020, 8, 467. (Doi:10.3390/biomedicines8110467)

• Shunsuke Tsukagoshi, Rumi Mikami, Kenta Arai*, Basic amino acid conjugates of 1,2‐diselenan‐4‐amine with protein disulfide isomerase‐like functions as a manipulator of protein quality control, Chem. Asian J. 2020, 15, 2646–2652. (Selected as Very Important Paper and front cover picture)

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2019

• Kenta Arai, Takahiko Matsunaga, Haruhito Ueno, Nozomi Akahoshi, Yuumi Sato, Gaurango Chakrabarty, Govindasamy Mugesh*, Michio Iwaoka*, Modeling Thioredoxin Reductase-like Activity Using Cyclic Selenenyl Sulfides: Participation of an NH...Se Hydrogen Bond through Stabilization of the Mixed Se–S Intermediate, Chem. Eur. J., 2019,25,12751–12760.(Selected as Cover Feature of the issue)

• Kenta Arai,* Yuui Osaka, Masahiro Haneda, Yuumi Sato,  Cyclic telluride reagents with remarkable glutathione peroxidase-like activity for purification-free synthesis of highly pure organodisulfides, Catal. Sci. Technol., 2019, 9, 3647-3655. (Selected as back-cover picture of the issue)

• Shunsuke Okada†, Motonori Matsusaki† , Kenta Arai, Yuji Hidaka, Kenji Inaba, Masaki Okumura and Takahiro Muraoka*, Coupling effects of thiol and urea-type groups for promotion of oxidative protein folding, Chem. Commun., 2019, 5, 759-762.

• Kenta Arai, Michio Iwaoka* Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide, Chapter 14 in Functional Disulfide Bonds, (Ed. Philip Hogg), Methods in Molecular Biology, Springer (2019).

 

2018

• 荒井堅太、岩岡道夫「インスリンの高次構造形成過程の解明－インスリン製剤の簡便な化学合成への展開」公益社団法人日本薬学会、ファルマシア、54、1135-1139、pp. 1135-1139、2018年 12月

• 荒井堅太、岩岡道夫「インスリンの簡便な化学合成法の開発－未来の糖尿病治療への期待」化学同人、化学、十月号、pp. 29～33、2018年10月

• Kenta Arai†, Toshiki Takei†, Reina Shinozaki, Masato Noguchi, Shouta Fujisawa, Hidekazu Katayama, Luis Moroder, Setsuko Ando, Masaki Okumura, Kenji Inaba, Hironobu Hojo*, Michio Iwaoka*, Characterization and Optimization of Two-Chain Folding Pathways of Insulin via Native Chain Assembly, Communications Chemistry, 2018, 1, Article number: 26. †Contributed equally to this work

• Kenta Arai, Haruhito Ueno, Yuki Asano, Gaurango Chakrabarty, Shingo Shimodaira, Govindasamy Mugesh*, Michio Iwaoka*, Protein Folding by Water-Soluble Cyclic Diselenides with Novel Oxidoreductase and Isomerase Activities. ChemBioChem, 2018, 19, 207-211.

• Prachi Verma, Amit Kunwar, Kenta Arai, Michio Iwaoka, K. Indira Priyadarsini, Mechanism of radioprotection by dihydroxy-1-selenolane (DHS): Effect of fatty acid conjugation and role of glutathione peroxidase (GPx), Biochimie, 2018, pp. 122-133.

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2017

• Michio Iwaoka*, Kenta Arai, Non-bonded Selenium•••Heteroatom Interactions in Selenoenzyme Glutathione Peroxidase and Mimics, Chapter 7 in Organoselenium Compounds in Biology and Medicine: Synthesis, Biological and Therapeutic Treatments, (Eds. Vimal Kumar Jain and K. Indira Priyadarsini), The Royal Society of Chemistry (2017).

• Kenta Arai*, Setsura Ueda, Sayaka Tamura, Rapid Electrochemical Conversion of Thiol and Disulfide into Difluoro- and Trifluoromethyl Thioethers in a Microfluidic Reactor. Curr. Green Chem., 2017, 4, pp. 137–143.

• Shingo Shimodaira, Yuki Asano, Kenta Arai, and Michio Iwaoka*, Selenoglutathione Diselenide: Unique Redox Reactions in the GPx-Like Catalytic Cycle and Repairing of Disulfide Bonds in Scrambled Protein, Biochemistry, 2017, 56, pp. 5644–5653.

• 荒井堅太 「セレン原子が切り開く、インスリン化学合成と機能の向上を目指した新しい道筋」、Acadamist Journal、 (2017)（ウェブはこちらからアクセスできます。）

• Kenta Arai†, Toshiki Takei†, Masaki Okumura†, Satoshi Watanabe†, Yuta Amagai, Yuya Asahina, Luis Moroder, Hironobu Hojo*, Kenji Inaba*, Michio Iwaoka* Preparation of Selenoinsulin as a Long-Lasting Insulin Analog, Angew. Chem. Int. Ed. 2017, 56, pp. 5522–5526.       †Contributed equally to this work

• Kenta Arai*, Ayako Tashiro, Yuui Osaka, Michio Iwaoka*, Glutathione Peroxidase-Like Activity of Amino-SubstitutedWater-Soluble Cyclic Selenides: A Shift of the Major Catalytic Cycle in Methanol. Molecules, 2017, 22.

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2016

• Prachi Verma, Amit Kunwar, Kenta Arai, Michio Iwaoka, K. Indira Priyadarsini, Alkyl chain modulated cytotoxicity and antioxidant activity of bioinspired amphiphilic selenolanes. Toxicol. Res. (Camb.), 2016, 5, pp. 434-445.

• Kenta Arai, Michio Iwaoka, Tetrahydroselenophene. A Unique Structural Motif for Biochemical Applications. Curr. Org. Chem. 2016, 20, pp.155-165. (DOI: 10.2174/1385272819666150724233306 )

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2015

• Michio Iwaoka, Nobukazu Sano, Ying-Yin Lin, Arisa Katakura, Masato Noguchi, Kohei Takahashi, Fumio Kumakura, Kenta Arai, Beena G. Singh, Amit Kunwar, K. Indira Priyadarsini, Fatty Acid Conjugates of Water-Soluble (±)-trans-Selenolane-3,4-diol: Effects of Alkyl Chain Length on the Antioxidant Capacity. ChemBioChem, 2015, 16, pp.1226-1234.

• Kenta Arai, Fumio Kumakura, Motoi Takahira, Natsumi Sekiyama, Nozomi Kuroda, Toshiki Suzuki, Michio Iwaoka, Effects of Ring Size and Polar Functional Groups on the Glutathione Peroxidase-Like Antioxidant Activity of Water-Soluble Cyclic Selenides. J. Org. Chem., 2015, 80, pp.5633-5642.

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2014

• Kenta Arai, Kenji Moriai, Akinobu Ogawa, Michio Iwaoka, An Amphiphilic Selenide Catalyst Behaves Like a Hybrid Mimic of Protein Disulfide Isomerase and Glutathione Peroxidase 7. Chem. Asian J., 2014, 9, pp.3464-3471. (DOI: 10.1002/asia.201402726) (Selected as back-cover picture of the issue)

• Kenta Arai, Thomas Wirth, Rapid Electrochemical Deprotection of the Isonicotinyloxycarbonyl Group from Carbonates and Thiocarbonates in a Microfluidic Reactor Org. Process Res. Dev. 2014, 18, pp.1377-1381.

• Kenta Arai, Kevin Watts, Thomas Wirth, Difluoro- and Trifluoromethylation of Electron-deficient Alkenes in an Electrochemical Microreactor. ChemistryOpen, 2014, 3, pp.23-28.

• Toshiki Takei, Yoshiko Urabe, Yuya Asahina, Hironobu Hojo, Takeshi Nomura, Kenichi Dedachi, Kenta Arai, Michio Iwaoka,Model, Study Using Designed Selenopeptides on the Importance of the Catalytic Triad for the Antioxidative Functions of Glutathione Peroxidase. J. Phys. Chem. B, 2014, 118, pp.492-500.

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2013

• Kenta Arai, Wataru Shibagaki, Reina Shinozaki, Michio Iwaoka, Reinvestigation of the Oxidative Folding Pathways of Hen Egg White Lysozyme: Switching of the Major Pathways by Temperature Control. Int. J. Mol. Sci., 2013, 14, pp.13194-13212. 

• Kenta Arai, Masato Noguchi, Beena G. Singh, K. Indira Priyadarsini, Katsuhiko Fujio, Yurika Kubo, Kyoko Takayama, Setsuko Ando, Michio Iwaoka, A Water-Soluble Selenoxide Reagent as a Useful Probe for the Reactivity and Folding of Polythiol Peptides. FEBS Open Bio, 2013, 3, pp.55–64.

• 岩岡道夫、荒井堅太「セレノタンパク質のフォールディング―ジセレニド結合を利用した効率的なフォールディングへの新手法」、化学同人、化学、一月号、pp. 66～67、2013年1月

• Michio Iwaoka, Kenta Arai, From Sulfur to Selenium. A New Research Arena in Chemical Biology and Biological Chemistry. Curr. Chem. Biol., 2013, 7, pp.2-24. (DOI: 10.2174/2212796811307010002)

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2012

• Kenta Arai, Fumio Kumakura, Michio Iwaoka, Kinetic and Thermodynamic Analysis of the Conformational Folding Process of SS-Reduced Bovine Pancreatic Ribonuclease A Using a Selenoxide Reagent with High Oxidizing Ability. FEBS Open Bio, 2012, 2, pp.60-70. 

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2011

• Sari Yoshida, Fumio Kumakura, Itsuki Komatsu, Kenta Arai, Yuko Onuma, Hironobu Hojo,* Beena G. Singh, K. Indira Priyadarsini, and Michio Iwaoka, Antioxidative Glutathione Peroxidase Activity of Selenoglutathione, Angew. Chem. Int. Ed. 2011, 50, pp. 2125–2128. 

• Kenta Arai, Kenichi Dedachi, Michio Iwaoka,* Rapid and Quantitative Disulfide Bond Formation for a Polypeptide Chain Using a Cyclic Selenoxide Reagent in an Aqueous Medium. Chem. Eur. J., 2011, 17, pp.481–485. (selected as VIP and Cover picture) (DOI: 10.1002/chem.201002742)

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2010

• Kenta Arai, Fumio Kumakura, Michio Iwaoka, Characterization of Kinetic and Thermodynamic Phases in the Pre-Folding Process of Bovine Pancreatic Ribonuclease A Coupled with Fast SS Formation and SS Reshuffling. Biochemistry, 2010, 49, pp.10535–10542.